Fourteen conferences were awarded automatic qualification for the 2025 championship. The five remaining berths were reserved for five at-large candidates. At-large consists of institutions from automatic-qualifying conferences that are not the conference champion and any remaining independent institutions.

Cal Lutheran (21-5), Juniata (29-4), Messiah (25-3), Springfield (22-4) and Vassar (22-6) were the five At-Large selections.